Prognostic Value of CD14HLA-DR Myeloid Derived Suppressor Cells in Diffuse Large B Cell Lymphoma / 中国实验血液学杂志

OBJECTIVE@#To investigate the role of myeloid-derived suppressor cells (MDSC) in the prognosis of patients with diffuse large B cell lymphoma (DLBCL).@*METHODS@#The peripheral blood of 52 DLBCL patients and 30 healthy volunteers was collected. The CD14HLA-DR was used as the immune marker for MDSC. The role of MDSC in the prognosis of DLBCL patients was analyzed by combination with the related clinicopathological data.@*RESULTS@#The proportion of MDSC in peripheral blood of newly diagnosed DLBCL patients increased significantly (P＜0.01). The expression of MDSC in DLBCL patients was related with clinical staging, lactate dehydrogenase (LDH) level and IPI score (P＜0.01). There was no significant correlation with sex, age, and B symptoms. Univariate analysis showed that the clinical stage, serum LDH level, IPI score and MDSC level were the adverse factors affecting the overall survival (OS). Multivariate analysis showed that IPI score and MDSC level were independent risk factors for OS in DLBCL patients.@*CONCLUSION@#MDSC can be used as an important index to evaluate the prognosis of DLBCL patients, contributing to evaluate the immune and tumor microenvironment of DLBCL patients.

Rapid Detection of Trace Volatile Organic Compounds By Electric Driven Cryo-Trap Movable Mass Spectrometer / 分析化学

An electric driven cryo-trap enrichment apparatus for the movable mass spectrometer was designed to realize rapid and automatic detection of trace volatile organic compounds (VOCs) in the atmosphere. Compared with traditional cryo-trap with refrigerant,the lowest temperature of cryocooler was down to-196℃, and it was easy to carry due to its small size, light weight and refrigerant-free. Cryo-trap combined with time-of-flight mass spectrometer was used to analyze benzene,toluene and xylene,and the result showed that the signal strength of benzene,toluene and xylene,with the cryo-trap enrichment,was improved by 212-fold, 254-fold and 242-fold,and the recoveries reached 98%,87% and 87%,respectively. The analytical time for single sample was only 14 min. The electric driven cryo-trap combined with on-line mass spectrometer was used to detect 39 kinds of VOCs listed in TO-14 method of EPA and ambient air, and the sensitivity of the newly designed instrument was better than the threshold of national indoor VOCs. The combination of electric driven cryo-trap and time-of-flight mass spectrometer could be used for rapid enrichment and monitoring of trace VOCs in the atmosphere and indoor air, showing great potential in in-site measurement and real-time monitoring of volatile organic pollutants.